The fungus Aspergillus oryzae is in microbes that have accumulated high lipids (oleaginous microorganism) has the ability to create and accumulate lipids within a cell in a high volume over 40% of the dry biomass. Depending on the culture conditions A. oryzae fermentation is vital in the food industry. He also has drawn attention to a substance that is important and highly valued species such as enzyme essential polyunsaturated fatty acids. Because A. oryzae fungus is safe. You can use a variety of substrates. There is growing quickly. A gene transfer system (Transformation system) and gene expression (gene expression), which facilitates the development of effective techniques of molecular species A. oryzae fungi because the fungi that contain fiber. In the process, fed with liquid (submerge cultivation) fiber mat or often called the pellet morphology (morphology) of this nature affect the nutrients and oxygen to the fungus. Because the fungus inside the pellet to get nutrients and oxygen throughout which affect the yields of interest. It is reported that when the fungus in liquid medium with the addition of beads. (Microparticle) cells to grow and accumulate lipids higher. Due to growth in the short fibers are dispersed (dispersed mycelia) cells receive oxygen and nutrients thoroughly. But how to fill microparticle Into food, thus increasing the cost of production. Therefore, genetically modified to mold a morphology that technique in molecular biology have gained attention. The report found that a defect in a gene Chitin synthase B (ChsB) by a gene associated with the synthesis of chitin. Which is an important component of cell walls. Affect the morphology of fungi. Cause mold to grow in a way that short fibers. So, in this work, with attention to genetically modified fungus A. oryzae to have impaired the function of the gene for mutant ChsB will be used as a source for the production of fatty acids that are highly valued next. To inhibit the function of genes required Plas Mick specific sequences of genes which have the desired deterrent on the plasmid. Flanked by some of the genes that were selected in the trans Falls Mansfield. (Trasformant) In this work, creating plasmids for use in the inhibition of the Ras gene ChsB A. oryzae technique yeast assembly process by homologous recombination in yeast Saccharomyces cerevisiae helps to connect the pieces. ChsB gene into a specific gene selection. The technique is simple, fast and does not require specific enzymes cut (restriction enzyme) and ligase enzymes to cut DNA and connecting pieces. Webcomic created by Plas this is done to take into A. oryzae fungus to Feng ChsB next to the function of genes.
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