Remaining from the previous step has been spinning with 30 volume 0.5 M acid for a period of 1 minute and 24-hour time, stir the solution of warm (enzyme/tissue ratio 1: 100) was added, and another 24-hour scramble to keep.
Remaining from the previous step has been blended with 30 volumes 0.5M acid and stir for 1 minute, 24 hours a solution of protease (enzyme / tissue ratio 1:100) was added and kept in order. stirred 24 hours
The rest of the previous steps have been blended with 30 books 0.5M acid for 1 minutes and stirring time 24 hours, solving the problem of the gastric juice. (the enzyme / tissue ratio 1: 100) was recorded in this and keep to stir 24 hours.