The formula of food feed fuel to low cost and appropriate production conditions, the enzyme from B. subtilis protoplasts rati was increasing with the appropriate statistical experimental planning. Plackett-Burman design help in the selection of the main factors for Response surface; RSM is a set of statistical and mathematical techniques that are useful for modelling and analyzing the responses. I found that both MgSO4 7H2O, K2HPO4, pH and time • Have a significant effect on the production of protease enzymes? Box-Behnken-experimental plan was designed to determine the appropriate concentration levels of the factor. Select three factors is MgSO4 7H2O K2HPO4, and two education pH • level that is (1,-1) and made a study of the effect of the planned time to experiment. Can produce the enzyme protease, at most, 50.13 Unit/ml which is closest to the value expected from statistical models, 50.84 Unit/ml caused soybean 2.40 g K2HPO4, 0.60? G MgSO4 7H2O, pH 7.0 and 0.80 g • at the cost price is 1.65 baht which Nutrient Broth recipe, there are standard costs stood at 2.82 baht per 100 ml fuel raising food production model that uses appropriate, which can produce the enzyme protease as much as possible. Nutrient Broth versus 0.8 grams and ml/Unit is 24.00 soybean meal 2.4 g Unit/ml. 29.60 where the success from the use of nitrogen source, low-cost.
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