Abstract Selaginella, an extant gen

Abstract
Selaginella, an extant genus of primitive
vascular plants, has survived over 400 million years of
evolution. In vitro morphogenesis in Selaginella microphylla
is considered for the first time to establish a welldocumented
aseptic culture on half- strength Murashige
and Skoog’s basal medium with 2ip (4.92–49.21 lM), or
Kn (4.65–46.47 lM) or GA3 (2.89–28.90 lM) for shoot
multiplication, and with different concentrations of IBA
(4.9–49 lm) to initiate root cultures. GA3 was instrumental
for shoot multiplication as well as induction of reproductive
structures in each and every leaf axil. On the other
hand, it is observed that IBA alone in S. microphylla can
act as signal molecules for induction of enormous numbers
of root masses from a few existing roots. An interesting
pattern of re-differentiation has also been observed where
apical portions of large numbers of roots were converted to
green shoot apical meristems. Further differentiation produced
tiny green shoots. Distinct bipolarity was noted in
shoots when they were isolated from root masses and
appeared as embryo-like structures. Chromosome analysis
from in vitro sporophytic plants revealed 2n = 16 chromosomes,
indicating chromosomal stability. The interesting in
vitro pattern of morphogenesis obtained in S. microphylla
may provide new insights into totipotency of plants.

ผลลัพธ์ (อังกฤษ) 2:

Abstract
Selaginella, an Extant genus of Primitive
Plants vascular, has survived over 400 million years of
Evolution. In vitro morphogenesis in Selaginella microphylla
is considered for the First time to establish a Welldocumented
Aseptic Culture on Half strength Murashige
and Skoog's basal Medium with 2ip (LM 4.92 to 49.21), or
Kn (LM 4.65 to 46.47) or GA3 (2.89 to 28.90. LM) for shoot
Multiplication, and with different concentrations of IBA
(4.9-49 LM) to initiate root Cultures. GA3 was instrumental
as well as induction of Multiplication shoot for Reproductive
Structures in each and every Leaf axil. On the Other
Hand, it is observed that IBA alone in S. microphylla Can
Act as Signal molecules for induction of enormous Numbers
of root masses from a few existing Roots. An Interesting
Pattern of re-differentiation has also been observed where
apical Portions of Large Numbers of Roots were converted to
shoot apical meristems Green. Further differentiation produced
tiny Green shoots. Distinct bipolarity was noted in
shoots when they were isolated from root masses and
appeared as embryo-like Structures. Analysis chromosome
from in vitro 2n = 16 chromosomes revealed Sporophytic Plants,
Indicating chromosomal stability. Interesting the in
vitro morphogenesis Pattern of S. microphylla obtained in
May provide insights Into New Totipotency of Plants.