In order to overcome the problems o

In order to overcome the problems of traditional T vectors, a T vector pQE-T based on pQE-30Xa was designed for directional cloning and prokaryotic expression. pQE-T can be used to clone PCR products directly. After the transformation and the extraction of the plasmids, the direction of insert can be determined by BamHI digestion.Here, fusion protein of 14-3-3 and ZsGreen (14-3-3-ZsGreen)and retinol binding protein from Homo sapiens (hRBP) can be successfully cloned and expressed.

In order to overcome the problems of traditional T vectors, a T vector pQE-T based on pQE-30Xa was designed for directional cloning and prokaryotic expression. pQE-T can be used to clone PCR products directly. After the transformation and the extraction of the plasmids, the direction of insert can be determined by BamHI digestion.Here, fusion protein of 14-3-3 and ZsGreen (14-3-3-ZsGreen) and retinol binding protein from Homo sapiens (. hRBP) can be successfully cloned and expressed.

In order to overcome the problems of traditional, T vectors a T vector pQE-T based on pQE-30Xa was designed for directional. Cloning and prokaryotic expression. PQE-T can be used to clone PCR products directly. After the transformation and the extraction. Of the plasmids the direction, of insert can be determined by, BamHI digestion.HereFusion protein of 14-3-3 and ZsGreen (14-3-3-ZsGreen) and retinol binding protein from Homo sapiens (hRBP) can be successfully. Cloned and expressed.
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