Adapted from the methods of Hamid and faculty (29) mix each signature peptides through the migration techniques to prepare ultra Phil concentration to the concentration DPPH solution 0.1 modular holiday. In ethanol concentration 95.5 percent in the ratio 1:4 for a period of 15 minutes and curing measure to absorb light at 517 nm wavelength. By testing a sample of 3 after it made a calculation of the percentage elimination of free radicals by calculating a minimum concentration can eliminate free radicals is 50 per cent (IC50), which is an acid (ascorbic acid), crisis core hotel as eliminating free radicals that is used as the positive control be calculated according to a formula.Scavenging effect (%) = [1- (A sample - A blank) / A control] × 100Note: A sample of a suck up, extract DPPH solution. A blank value to absorb light of the extract DPPH solution cannot fill.A control is the value of a solution absorb light that does not fill the extract DPPH.
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