Crosslinking
The fibers were crosslinked in glutaraldehyde vapor at 80 ◦C
for 12 h, and heated at 120 ◦C for another 12 h to remove residual
glutaraldehyde.
2.4.2. In vitro Ag release
The AgNPs/PVA/CM-chitosan nanofibers (approximately 10 mg)
were immersed in 50 mL phosphate buffer solution (PBS) after
crosslinking. The samples were incubated at 37 ◦C and stirred
at 90 rpm. After digested by HNO3, the Ag concentration of the
solution was determined by Inductively Coupled Plasma Atomic
Emission Spectroscopy (ICP-AES) (PROFILE SPEC, Leeman, USA).