ระดับคะแนน 4 หมายถึง ประสิทธิภาพระด

ระดับคะแนน 4 หมายถึง ประสิทธิภาพระดับมาก

ผลลัพธ์ (อังกฤษ) 1:

Level 4 level performance means so much

ผลลัพธ์ (อังกฤษ) 2:

4-star rating means a high level of performance.

ผลลัพธ์ (อังกฤษ) 3:

PCR analysis.DNA was isolated from GUS positive plants using CTAB method [15]. PCR screening of putative transformants was performed. Using specific primers for Cry 2Ab gene. The plasmid was used as a positive control. The sequences of forward and reverse. Primers were 5 'CATTCAGCTTCCAGCACAAGAGCC3'; and 5 'TGGGTGCCAGAGTTCAGGGTCACG3', respectively. The PCR mixture were performed. In a total volume of 25 thermal L (1.0 thermal l of 10, mM dNTPs 5.0 thermal L 10 × buffer containing MgCl2, 2.0 thermal L, of 50-ng / thermal L, template DNA 0.4 thermal L. Of, Taq polymerase 1.0 thermal l of 70 pmol from each primer and 14.6 thermal l sterile distilled water). Amplification is accomplished. Through a Thermal, Cycler (Biometra Germany), using first one incubation at 94 ° C for 5 min and the step cycle program set. To denaturant at 94 ° C for 30 s to anneal, at 56 ° C for 50 s and then extended at 72 ° C for 75 s for a total of 30 cycles;? After that an extra, step of extension at 72 ° C for 10 min was performed.