This research is preparing a hybrid film darawang polymer pigeon Henrietta Knapp polymer ethylene amine นอิ (PPy-PEI hybrid film) to prepare a biological sensor for measuring concentration of urea synthetic PPy film (Polypyrrole) on stainless steel electrode with cyclic kawo-Tammy email Tri-then improve the face film PPy to suit PIN s polyurethane enzymes through the creation of Covalent bonds by film coating in PEI hybrid delivers film experiments. 2. How was the introduction of steam curing PPy film of an EDA (Ethylenediamine) (Vapor deposition) and dipped into a solution of EDA PPy film (casting Solution) then you take the PPy film absorbs the EDA, and then go into the process of Plasma polymerization and leads to enzyme immobilization. Polyurethane s through the creation of Covalent bonds with glutamine Raman aldehyde. It found that the hybrid film preparation can be measured response to urea by the relationship is linear in the range of concentration of urea, 5 × 10-4 to 5 × 10-2 M (1.3-3.3 pUrea) by film prepared by EDA technique of slurry in the urea, and to respond better. In response to urea 42.08 mV/pUrea (R2 = 0.9775) uses the response time for 30 seconds, then remove the hybrid film preparation derived elements on the surface, with advanced techniques XPS XPS spectrum finds appear peak – N = (imine) that has apparently caused the creation of Covalent bonds of glutamine fungal enzyme aldehyde with polyurethane s.
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