Alginate solution (1.0, 1.5, 2.0, 2

Alginate solution (1.0, 1.5, 2.0, 2.5, 3.0 and 4.0% (w/v)) was
prepared by dissolving sodium alginate in deionized water containing
a certain amount of laccase (200e800 mg L1). Then
different ratios of enzyme/alginate (E/A) (v/v) were mixed under
shaking (Baysal, 2007). The mixture was dropped by means of a
syringe into a CaCl2 solution (1e4% w/v) under shaking. After 1 h
the beads (about 3e4 mm in diameter) were collected from the
solution and washed with CaCl2 0.5% (w/v) twice and then washed
three times with deionized water. The filtered hardening solutions
and the two washings were collected for loading efficiency (Eq. (1))
and immobilization yields determination (Eq. (2)). Loading efficiency
and immobilized yield were defined as the percentage of
total enzyme entrapped and the specific activity ratio of entrapped
laccase to free laccase, respectively.

ผลลัพธ์ (อังกฤษ) 2:

Solution alginate (1.0, 1.5, 2.0, 2.5, 3.0 and 4.0% (W / v)) was
Prepared by dissolving Sodium alginate in Deionized Water containing
a Certain amount of Laccase (200e800 mg L? 1). Then
different ratios of enzyme / alginate (E / A) (v / v) were mixed under
shaking (BAYSAL, 2,007th). The mixture was dropped by means of a
Syringe Into a CaCl2 Solution (1E4% W / v) under shaking. After 1 h
The Beads (About 3e4 mm in Diameter) were Collected from The
Solution CaCl2 and washed with 0.5% (W / v) and then washed Twice
Three Times with Deionized Water. The filtered quenching Solutions
and The Two washings were Collected for loading efficiency (Eq. (1))
and immobilization yields determination (Eq. (2)). Loading efficiency
and yield were immobilized defined As The percentage of
total enzyme entrapped and Specific Activity The ratio of entrapped
Laccase to free Laccase, respectively.