Semen 1 ml. 15° C centrifuge spinning at 8000 rpm, 5 minutes. Dispose of transparent partsAdd 5% sodium hypochlorite 0.5 ml. and mixIncubate at 37˚C 1 hrat 15˚C 8000 rpm 5 minsKeep the decant supernatant Wash with distilled water and ethanal 95%at 15˚C 8000 rpm 5 mins.
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