Transgenic miceThe characterization

Transgenic mice
The characterization of 3xTg-AD mice has been described previously (Oddo et al., 2003). Briefly, two independent transgenes encoding human APPSwe and the human tauP301L (both under control of the mouse Thy1.2 regulatory element) were co-microinjected into single- cell embryos harvested from homozygous mutant PS1M146V knockin (PS1-KI) mice (Oddo et al., 2003). We crossed the 3xTg-AD mice with tau knockout mice generated in the laboratory of Dr. Vitek (Dawson et al., 2001). Through careful breeding, we derived a colony of 3xTg- AD mice that lack both copies of the endogenous mouse wild type tau gene (i.e., hPS1KIM146V/hPS1KIM146V; hAPPSWE/hAPPSWE; hTauP301L/ hTauP301L; mTau−/−; where h = human and m = mouse gene). We refer these mice as the 3xTg-AD/mtauKO. In this work, 18 months of age homozygous Non-transgenic (Ntg), tau knockout (mtauKO), 3xTg- AD and 3xTg-AD/mtauKO, 16–20 per group (males and females) was used. Both 3xTg-AD and 3xTg-AD/mtauKO mice were homozygous for APP and tau transgenes. Mice (Ntg, mtauKO, 3xTg-AD and 3xTg-AD/ mtauKO) have the same genetic background (hybrid 129/C57BL6 back- ground). All animal procedures were performed in accordance with the National Institutes of Health and University of California guidelines and Use Committee at the University of California,

ผลลัพธ์ (อังกฤษ) 3:

Transgenic The characterization of mice 3 xTg - AD mice has been described previously (Oddo et al ., 2003). Briefly, two independent transgenes encoding the human and human APPSwe tauP L 301 (both under the control of mouse Thy 1.2 regulatory element) were co-microinjected into single-cell embryos harvested from homozygous mutant PS M 1 146 V knockin (PS 1-KI) mice (Oddo et al ., 2003).3 We crossed the xTg - AD tau mice with knockout mice generated in the laboratory of Dr.Vitek (Dawson et al ., 2001). Through careful breeding, we derived a colony of 3 xTg - AD mice that lack both copies of the endogenous wild type mouse tau gene (i. e., hPS 1 146 KIM V/hPS KIM 1 146 V ; hAPPSWE/hAPPSWE ; hTauP L 301 / 301 hTauP L ; mTau −/−; where H=human M=mouse and gene).refer We these mice as the 3 xTg - AD/mtauKO.In this work, 18 months of age homozygous Non-transgenic (Ntg), tau knockout (mtauKO), 3 xTg - AD and 3 xTg - AD/mtauKO, 16 - 20 per group (males and females) was used. Both xTg 3 - 3 AD and xTg - AD/mtauKO mice were homozygous for APP and tau transgenes. Mice (Ntg, mtauKO,3 xTg - AD and 3 xTg - AD/mtauKO) have the same genetic background (hybrid 129/C BL 6 57 back-ground) .All animal procedures were performed in accordance with the National Institutes of Health and University California of guidelines and at the University Use Committee of California,