Cysteine rich intestinal protein (C

Cysteine rich intestinal protein (CRIP) is an 8.5 kD protein which contains a
distinct LIM domain consisting of two zinc coordinating fingers in close proximity to one
another. There are several facts known about CRIP, but the function of the protein is still
not well understood. CRIP mRNA is most abundant in macrophages, PBMCs (peripheral
blood mononuclear cells), and the intestine. It is also very abundant in thymus, spleen,
heart, and lungs. Overexpression of CRIP in transgenic mice leads to increased secretion
of Th2 (T helper 2) cytokines (interleukin 6, IL-6 and interleukin 10, IL-10) and
decreased secretion of Th1 (T helper 1) cytokines (interferon-γ, IFN- γ and interleukin-2,
IL-2) after challenge of mice with LPS (lipopolysaccharide) or virus, or mouse
splenocytes with PHA (phytohemagglutinin). In order to better understand the function
of CRIP we designed a CRIP affinity purification column to identify possible protein
partners. Using the column we identified calreticulin (CRT) as a possible protein partner.
CRT was first identified as a calcium binding chaperone in the endoplasmic reticulum, but recently, CRT has been shown to be necessary for cell adhesion and spreading
because of its ability to bind integrins in the cell membrane and regulate integrin-
mediated cell adhesion. CRT has also been localized to the nucleus where it binds to
nuclear steroid hormone receptors, inhibiting their ability to bind to DNA. In order to
further explore the relationship between CRIP and CRT, their mRNAs were measured
during RAW 264.7 cell proliferation and upon treatment of cells with LPS +/- IFN-γ
which led to apoptosis in this cell type. During cell proliferation both CRIP and CRT
mRNA expression increased significantly and upon treatment with LPS +/- IFN-γ mRNA
expression of both proteins decreased significantly. Immunocytochemistry using
confocal microscopy revealed that both proteins colocalized during cell proliferation.
The binding of CRIP to CRT may shift localization of CRT from the endoplasmic
reticulum to sites of cell adhesion and/or the nucleus. Since CRT is an important
chaperone for IFN-γ (a Th1 cytokine) and MHC-I (major histocompatibility 1)
complexes, this may also explain why overexpression of CRIP leads to increased
secretion of Th2 cytokines and decreased expression of Th1 cytokines.

ผลลัพธ์ (แอฟริกา) 1:

Sisteïen ryk derm proteïen (Crip) is 'n 8.5 KD proteïen wat bevat 'n.
Duidelike LIM domein bestaande uit sink koördinering van twee vingers in die nabyheid van Een.
ander. Daar is verskeie bekende feite oor Crip, maar die funksie van proteïen is die stil.
nie goed verstaan ​​nie. Die meeste Crip mRNA is volop in makrofage, PBMCs (Perifere.
Bloed Mononukleêre selle), en die dunderm. Dit is ook baie volop in Thymus, Spleen ,.
hart en longe. Expressie van Crip in transgeniese muise lei tot verhoogde afskeiding.
van Th2 (T Helper 2) sitokiene (interleukin 6, IL-6 en interleukin 10, IL-10) en.
Afname afskeiding van Th1 (T Helper 1) sitokiene (interferon-gamma, IFN. - gammastrale en interleukin-2 ,.
IL-2) Na Challenge van die muise met LPS (lipopolysaccharide) of Virus, of muis.
Splenocytes met PHA (phytohemagglutinin). Om beter te verstaan ​​die funksie.
Ontwerp van 'n Crip Crip Ons Affinity Suiwering kolom Moontlike proteïen te identifiseer.
Partners. Die gebruik van die rubriek Ons geïdentifiseer calreticulin (CRT) as 'n moontlike proteïen Partner.
CRT is die eerste keer wat geïdentifiseer is as 'n kalsium-bindende Chaperoneweg in die endoplasmiese retikulum, maar onlangs het CRT getoon wat nodig is vir Cell adhesie te wees en versprei.
As gevolg van sy vermoë om te bind Integrines in die selmembraan en reguleer Integrin-.
bemiddel Cell adhesie. CRT is ook gelokaliseerde aan die kern waar dit bind aan.
kern hormoon steroïde reseptore, inhiberende hul vermoë om te bind aan DNA. Ten einde te
verder te verken Die verhouding tussen Crip en CRT, is hul mRNAs gemeet.
tydens RAW 264,7 selproliferasie en Behandeling van selle met LPS +/- op IFN-gamma.
wat gelei het tot apoptose in hierdie tipe sel. Tydens Crip Beide selproliferasie en CRT.
mRNA Expression aansienlik vermeerder op Behandeling met LPS en IFN-gamma mRNA +/-.
Afname Expression van Beide Proteïene aansienlik. Immunocytochemie gebruik
confocal mikroskopie die lig gebring dat Colocalized Beide Proteïene tydens selproliferasie.
Die binding van Crip te CRT mag verskuiwing van die endoplasmiese Lokalisering van CRT.
retikulum te Sites Cell adhesie en / of die kern. Sedert CRT is 'n belangrike
Chaperoneweg vir IFN-gammastrale ('n Th1 sitokiene) en MHC-I (Groot weefselverenigbaarheids 1).
komplekse, ook Dit kan verduidelik waarom expressie van Crip lei tot verhoogde.
afskeiding van Th2 sitokiene en verlaagde Expression van Th1 sitokiene.