ฉันรักการแปลwind blower for 10 - 15

ฉันรักการแปลwind blower for 10 - 15 min and blocked with PBS containing
2% ovalbumin and 1.5% sucrose. The membrane
was dried at 50oC under a dry wind blower for 10-15 min
and washed twice with 0.01 M, sodium phosphate buffer,
pH7.2 before drying it for 10 - 15 min at 50oC. A polyethylene
plastic sheet (26 × 8 cm) of 0.2 mm thickness was
coated with acrylic adhesive on one side and the 2.5 cm
wide membrane was placed centrally at a spacing of 1.5 cm
from the top and 4cm from the bottom end of the sheet.
The conjugate coated glass-fiber pad was placed on the
lower end of the membrane, so as to overlap 2mm on it. A
filter pad was placed to overlap 2 mm on the lower end of
the conjugate release pad to act as sample pad and another
pad (CF4) was placed to overlap 2 mm on the upper end of
the membrane to act as absorbent pad. The assembly was
cold-laminated using an 8 cm wide transparent adhesive
tape. The laminated 26 × 8 cm assembly was cut into lateral-
flow strips of 8 × 0.4 cm. The strips were stored in an
airtight plastic bottle containing a desiccant pack.
Extraction of TBSV-infected tomato
Tomato leaf tissues infected by TBSV were extracted
with 5 mL of 0.5X PBS (pH7.4) solution using tissue
grinder (Agdia, USA). Then the crude sap was precipitated
briefly and the supernatant was used for specificity of
RIGS kit.
RESULTS AND DISCUSSION
TBSV-B was successfully purified from leaf tissues of
N. clevelandii, showing denatured capsid proteins of
TSWV-KP were detected on SDS-PAGE gel (Fig. 1(c) and
data not shown). The purified TBSV-B particle is about
35 nm. Polyclonal sera specific to TBSV were produced
from rabbit and the IgG specific to TBSV was further purified
using Protein A affinity chromatography from sera.
The concentration of TBSV-IgG was 1.0 mg/mL. In the
production of AuNPs conjugated with TBSV-IgG, the particle
size is inversely proportional to the sodium citrate
volume. In the TBSV-IgG antibody-AuNP conjugation,
the antibody was absorbed on the AuNP surface. The
effects of pH values on the conjugation were studied by
evaluating the absorbance between 400nm and 650 nm.
Also, the main purpose of the assay was to allow visual
evaluation, so it was only used as a qualitative assay to
detect contamination at a threshold level. As the Na2CO3
concentration increased, the maximum absorption wavelength
increased up to the optimal concentration and then
decreased (data not shown). The definition of the optimal
concentration of TBSV-antibody was the one that gave the
required visibility and the best sensitivity. During antibody
concentration optimization, the minimal stable polyclonal
IgG concentration form antibody-AuNP conjugation was
firstly designed and evaluated (Fig. 2), and then the optimal
concentration was studied.

ผลลัพธ์ (อังกฤษ) 1:

I would love to translate the wind blower for 10-15 min and blocked with PBS containing.2% ovalbumin and 1.5% sucrose. The membranewas dried at 50oC under a dry wind blower for 10-15 minand washed twice with 0.01 M, sodium phosphate buffer,pH7.2 before drying it for 10 - 15 min at 50oC. A polyethyleneplastic sheet (26 × 8 cm) of 0.2 mm thickness wascoated with acrylic adhesive on one side and the 2.5 cmwide membrane was placed centrally at a spacing of 1.5 cmfrom the top and 4cm from the bottom end of the sheet.The conjugate coated glass-fiber pad was placed on thelower end of the membrane, so as to overlap 2mm on it. Afilter pad was placed to overlap 2 mm on the lower end ofthe conjugate release pad to act as sample pad and anotherpad (CF4) was placed to overlap 2 mm on the upper end ofthe membrane to act as absorbent pad. The assembly wascold-laminated using an 8 cm wide transparent adhesivetape. The laminated 26 × 8 cm assembly was cut into lateral-flow strips of 8 × 0.4 cm. The strips were stored in anairtight plastic bottle containing a desiccant pack.Extraction of TBSV-infected tomatoTomato leaf tissues infected by TBSV were extractedwith 5 mL of 0.5X PBS (pH7.4) solution using tissuegrinder (Agdia, USA). Then the crude sap was precipitatedbriefly and the supernatant was used for specificity ofRIGS kit.RESULTS AND DISCUSSIONTBSV-B was successfully purified from leaf tissues ofN. clevelandii, showing denatured capsid proteins ofTSWV-KP were detected on SDS-PAGE gel (Fig. 1(c) anddata not shown). The purified TBSV-B particle is about35 nm. Polyclonal sera specific to TBSV were producedfrom rabbit and the IgG specific to TBSV was further purifiedusing Protein A affinity chromatography from sera.The concentration of TBSV-IgG was 1.0 mg/mL. In theproduction of AuNPs conjugated with TBSV-IgG, the particlesize is inversely proportional to the sodium citratevolume. In the TBSV-IgG antibody-AuNP conjugation,the antibody was absorbed on the AuNP surface. Theeffects of pH values on the conjugation were studied byevaluating the absorbance between 400nm and 650 nm.Also, the main purpose of the assay was to allow visualevaluation, so it was only used as a qualitative assay todetect contamination at a threshold level. As the Na2CO3concentration increased, the maximum absorption wavelengthincreased up to the optimal concentration and thendecreased (data not shown). The definition of the optimalconcentration of TBSV-antibody was the one that gave therequired visibility and the best sensitivity. During antibodyconcentration optimization, the minimal stable polyclonalIgG concentration form antibody-AuNP conjugation wasfirstly designed and evaluated (Fig. 2), and then the optimalconcentration was studied.

ผลลัพธ์ (อังกฤษ) 2:

I love to translate Wind Blower for 10 - 15 min and Blocked with PBS containing
2% ovalbumin and 1.5% sucrose. The membrane
was Dried at 50oC under a Dry Wind Blower for 10-15 min
and washed twice with 00:01 M, Sodium phosphate buffer. ,
PH7.2 before Drying it for 10 - 15 min at 50oC. A polyethylene
Plastic Sheet (26 × 8 cm) of 0.2 mm thickness was
coated with acrylic adhesive on one Side and the 2.5 cm
Wide membrane was Placed centrally at a Spacing of. 1.5 cm
from the top and 4cm from the bottom End of the Sheet.
The conjugate coated Glass-fiber Pad was Placed on the
Lower End of the membrane, so as to Overlap 2mm on it. A
Filter Pad was Placed to Overlap 2 mm on. the Lower End of
the conjugate release Pad to Act as sample Pad and another
Pad (CF4) was Placed to Overlap 2 mm on the Upper End of
the membrane to Act as absorbent Pad. The Assembly was
cold-laminated using an 8 cm Wide transparent. adhesive
Tape. The laminated 26 × 8 cm Assembly was Lateral- Cut Into
Strips flow of 8 × 0.4 cm. The Strips were stored in an
airtight Plastic Bottle Pack containing a desiccant.
Extraction of TBSV-infected Tomato
Tomato Leaf tissues were infected by TBSV. extracted
with 5 mL of PBS 0.5x (PH7.4) Solution using tissue
Grinder (Agdia, USA). Then the SAP was precipitated Crude
briefly and the supernatant was used for specificity of
rigs Kit.
RESULTS AND DISCUSSION
TBSV-B was successfully Purified. Leaf from tissues of
N. Clevelandii, showing denatured capsid Proteins of
TSWV-SDS-PAGE gel on KP were detected (Fig. 1 (C) and
Data not shown). The Purified TBSV-B particle is About
35 NM. Polyclonal Sera specific. to TBSV were produced
from Rabbit and the IgG specific to TBSV was further Purified
using Protein A affinity chromatography from Sera.
The concentration of TBSV-IgG was 1.0 mg / mL. In the
Production of AuNPs conjugated with TBSV-IgG, the particle
Size is. inversely proportional to the Sodium citrate
volume. In the TBSV-IgG antibody-AuNP conjugation,
the antibody was absorbed on the AuNP surface. The
effects of pH values ​​on the conjugation were studied by
evaluating the absorbance between 400nm and 650 NM.
Also, the. Main purpose of the assay was to Allow Visual
evaluation, so it was only used as a qualitative assay to
detect contamination at a threshold level. As the Na2CO3
concentration Increased, the maximum absorption wavelength
Increased up to the Optimal concentration and then
Decreased (Data not. shown). The Definition of the Optimal
concentration of TBSV-antibody was the one that Gave the
required visibility and the Best sensitivity. During antibody
concentration Optimization, the Minimal Stable polyclonal
IgG antibody concentration-form AuNP conjugation was
firstly designed and evaluated (Fig. 2), and then the Optimal
concentration was studied.

ผลลัพธ์ (อังกฤษ) 3:

I love wind translation blower for 10 - 15 min and blocked with PBS containing
2% ovalbumin and 1.5% sucrose. The membrane
was. Dried at 50oC under a dry wind blower for 10 - 15 min
and washed twice with 0.01 M sodium, phosphate, buffer
pH7.2 before. Drying it for 10 - 15 min at 50oC. A polyethylene
plastic sheet (26 × 8 cm) of 0.2 mm thickness was
.Coated with acrylic adhesive on one side and the 2.5 cm
wide membrane was placed centrally at a spacing of 1.5 cm
from. The top and 4cm from the bottom end of the sheet.
The conjugate coated glass-fiber pad was placed on the
lower end of the. Membrane so as, to overlap 2mm on it. A
filter pad was placed to overlap 2 mm on the lower end of
the conjugate release. Pad to act as sample pad and another
.Pad (CF4) was placed to overlap 2 mm on the upper end of
the membrane to act as absorbent pad. The assembly was
cold-laminated. Using an 8 cm wide transparent adhesive
tape. The laminated 26 × 8 cm assembly was cut into lateral -
flow strips of 8 × 0.4, cm. The strips were stored in an
airtight plastic bottle containing a desiccant pack.
Extraction of TBSV-infected tomato
.Tomato leaf tissues infected by TBSV were extracted
with 5 mL of 0.5X PBS (pH7.4) solution using tissue
grinder (Agdia,, USA). Then the crude sap was precipitated
briefly and the supernatant was used for specificity of
RIGS kit.
RESULTS AND. DISCUSSION
TBSV-B was successfully purified from leaf tissues of
N. Clevelandii showing denatured, capsid proteins of
.TSWV-KP were detected on SDS-PAGE gel (Fig. 1 (c) and
data not shown). The purified TBSV-B particle is about
35 nm. Polyclonal. Sera specific to TBSV were produced
from rabbit and the IgG specific to TBSV was further purified
using Protein A affinity. Chromatography from sera.
The concentration of TBSV-IgG was 1.0 mg / mL. In the
production of AuNPs conjugated, with TBSV-IgG. The particle
.