นำ C.gallica laccase ย้ายมาใน Semi-

นำ C.gallica laccase ย้ายมาใน Semi- solid- state fermentation หลังจากเติบโต 5 วันแล้ว, ต่อไป Hydrate ด้วย Minimum medium (MM) 15 mL แล้วปรับด้วย acetate buffer ( pH 5.0) จากนั้นนำไปเลี้ยงใน Malt extract agar (MEA) แล้วนำไป Supplemented with CuSO4, นำไปบ่มที่อุณหภูมิ 30 องศาเซลเซียส,Extract ด้วย NaOAC ( pH 5.0 ) และ Filtered and centrifuged at 4 °C สุดท้ายก็จะได้ Production of laccase.

ผลลัพธ์ (อังกฤษ) 1:

Apply C.gallica laccase in Semi-solid move-state fermentation after growing 5 days and then continue to Hydrate, with Minimum medium (MM) 15 mL, and then adjust with acetate buffer (pH 5.0) and then be raised on Malt extract agar (MEA) and Supplemented with CuSO4, leading to the curing temperature of 30 degrees Celsius, Extract with NaOAC (pH 5.0) and centrifuged and Filtered at 4° C, it is final. Production of laccase.

ผลลัพธ์ (อังกฤษ) 2:

The move comes in the C.gallica laccase Semi- solid- state fermentation, after growing 5 days ago, on to Hydrate with Minimum medium (MM) 15 mL, adjusted by acetate buffer (pH 5.0) were then cultured in Malt extract agar (. MEA), then Supplemented with CuSO4, incubated at 30 ° C, Extract with NaOAC (pH 5.0) and Filtered and centrifuged at 4 ° C final will Production of laccase.

ผลลัพธ์ (อังกฤษ) 3:

The C.gallica laccase moved up in the Semi - solid - state fermentation after growing 5 days, next Hydrate with Minimum medium. (MM) 15 mL and adjusted by acetate buffer (pH 5.0) and then cultured in Malt extract agar (MEA) was Supplemented with. CuSO4 was incubated at 30 C,,Extract with NaOAC (pH 5.0) and Filtered and centrifuged at 4 ° C finally will Production of laccase.


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