Post-extension evaluation of semen motility.This was done as described by Ajala et Al18 for.Pawpaw juice extender. Briefly the immediate,,Post-extension sperm cell progressive motility.68.Was assessed and recorded as zero hour motility.Score in percentage (%). Subsequent motility.Scores were done hourly for 6 hours, Each time.The assessment of sperm cell motility always.Started from D2 and continued in order, to D7And lastly D1 (100% buffer).The motility assessment was done quickly and.Carefully to ensure little time-lag in assessing.Other extended semen. The concentration of.Motile sperm cells of the extended semen was.Estimated for the most suitable extender using.The average pre-extension semen volume and.Concentration with average post-extension.Motility at 2 hours storage time and the, value.Recorded.Analysis of data.A descriptive analysis was performed by.Calculating means of sperm cell motility with.Results presented as mean edge standard error of.Mean (SEM). Using SPSS, 11 software one.Way ANOVA test at 5% significant level was,,,Used to determine whether or not there were.Significant differences between mean motility.Scores of sperm cells between the three.Comparable extenders at 2 and 3 hours after.Semen extension; and between these storage.Times (at room temperature) in the most superior.Extender.RESULTS.The semen characteristics of the white and black.Bucks are as shown in Table 1 (a), (b), (C and.)(d). The average motility score of sperm cells of.The white Buck was significantly higher.(P = 0.0001) than that of the Black Buck Table 1,,(A and b). Semen colour which is an indication.Of sperm cell concentration was consistently.Milky-white for the white buck while it was.Consistently watery for the Black Buck. This.Indicated poor sperm cell concentration of the.Black Buck. This was supported by the mean.Concentration of sperm cells of white buck.Which was significantly higher (P = 0.0001 than.)The Black Buck 's (Tables 1a and 1b).However there was, no statistical difference (P.0.321) between the live scores (%) of sperm.Cells of both bucks. The percentage of abnormal.Sperm cells in the white buck in contrast, to the.Black Buck ', s was less than 20% recommended.By Zemjanis19 (Tables 1c and 1D).PH of buffer.The pH of 2.5% sodium citrate in the 8 trials.Ranged from 8.5 to 9.0 with an average value of.8.7 edge 0.9.Values of coconut milk.The volumes of coconut milk obtained in the 8.Trials ranged from 9 ml to 24 ml with a mean.Value of 20.8 edge 1.4 ml. The pH of coconut milk.Ranged from 5.8 to 6.1 with an average value of.6.0 edge 0.3. The relative amounts of constituents.Of each extender and mean pH are as shown in.Table 2.Parameters of semen prior to extension.Evaluation of the undiluted semen before.Extension in the 8 trials is as shown in Table 3.Post-extension motility scores of semen.The values presented here are those of the 5.Trials used in the statistical analyses for up to 3.Hours post-extension (P.E.) of semen. They are.Shown in Table 4 (A and b) for 2 and 3 hours P.E.Respectively at room, temperature. In all the 8.Trials the motility, scores observed dropped to.Virtually zero value by 4 hours P.E. The results.Of the 4 hours and above are not shown. The.Motility scores at 2 hours after extension in D2.Were generally higher than in D3 and D4 in all.The trials except in, trial 5. It was also observed.That D3 was not different (P = 0.444) from D4 in.Preserving semen motility at 2 hours P.E.The motility scores at 3 hours after extension.Followed the same pattern of decreasing motility.Scores from extenders D2 to D4 as observed at 2.Hours P.E. (Tables 4A and 4B). No statistical.Difference (P = 0.693) was observed when mean.Sperm cell motility at 2 and 3 hours P.E. Were.Compared for D2. In addition mean motility,,Scores of semen in D2 at 3 hours P.E. Was not.Different (P = 0.106) from that in D3 at 2 hours.P.E. Using 52.6% mean sperm cell motility in.0.6 ml extended semen after, 2 hours we.Estimated concentration of motile sperm cells in.D2 as 161.5 x 106 sperm cells (i.e. 269.2 million.Sperm cells per.
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