The production conditions of cordyc

The production conditions of cordycepin, which is a kind of nucleoside analogue, were investigated by a surface culture using Cordyceps militaris NBRC 9787. And The effect of ferrous sulfate addition on production of cordycepin (3’ -deoxyadenosine) The optimal carbon/nitrogen ratio (w/w) was 2/1 when glucose was used as the carbon source. The lower medium depth resulted in a higher productivity over the present experimental range from 1.8 to 5.3 cm. Under the optimal conditions, the maximum cordycepin concentration in the culture medium reached 640 mg/l, and the maximum cordycepin productivity was 32 mg/(l•day). The results showed that the optimal addition condition was on day 0 with 1 g/L of ferrous sulfate, and the maximal amount of cordycepin reached 596.59±85.5 mg/L, about 70% higher than the control without ferrous sulfate addition. Meanwhile, the consumption of inosine 5 -monophosphate (IMP), a potential precursor of cordycepin, was decreased rapidly. Transcription levels of important genes encoding adenylosuccinate synthetase (purA), IMP cyclohydrolase (purH) and IMP dehydrogenase (guaB).

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The production conditions, of cordycepin which is a kind of nucleoside analogue were investigated, by a surface culture. Using Cordyceps militaris NBRC 9787. And The effect of ferrous sulfate addition on production of cordycepin (3 '- deoxyadenosine). The optimal carbon / nitrogen ratio (w / W) was 2 / 1 when glucose was used as the carbon source.The lower medium depth resulted in a higher productivity over the present experimental range from 1.8 to 5.3 cm. Under. The optimal conditions the maximum, cordycepin concentration in the culture medium reached 640 mg / L and the, maximum cordycepin. Productivity was 32 mg / (L - day). The results showed that the optimal addition condition was on day 0 with 1 g / L of ferrous. Sulfate.And the maximal amount of cordycepin reached 596.59 edge 85.5 mg / L about 70%, higher than the control without ferrous sulfate. Addition. Meanwhile the consumption, of inosine 5 - monophosphate (IMP), a potential precursor of cordycepin was decreased,, Rapidly. Transcription levels of important genes encoding adenylosuccinate synthetase (purA), IMP cyclohydrolase (purH). And IMP dehydrogenase (guaB).