The plasmids, pcDNA3, pcDNA3-IHNV-G

The plasmids, pcDNA3, pcDNA3-IHNV-G, pcDNA3-SHRV-G, and
pcDNA3-SVCV-G, were each used to transform competent Escherichia coli
JM109, and these transformed organisms were grown in the presence of ampicillin.
Large-scale preparations of each plasmid were made with the Qiagen
(Valencia, Calif.) plasmid purification kit, according to the manufacturer’s protocol.
The concentration of purified plasmids was determined by optical density
at 260 nm.

ผลลัพธ์ (แอฟริกา) 1:

Die plasmiede, PcDNA3, PcDNA3-IHNV-G, PcDNA3-SHRV-G en
PcDNA3-SVCV-G, is elk wat gebruik word om te transformeer bevoegde Escherichia coli
JM109, en dit omskep organismes gekweek in die teenwoordigheid van ampisillien.
Grootskaalse voorbereidings. gemaak van elke plasmied was met die Qiagen
(Valencia, Kalifornië.) plasmied Suiwering Kit, volgens protokol die vervaardiger se.
Die konsentrasie van gesuiwerde plasmiede is bepaal deur optiese digtheid
by 260 NM.