For bacterial fermentation in the first 70l be made in 5l size fermentation tanks will be used as fuel tanks for fermentation with a quantity of 70l work 50-L fungi culture is made 24 hours at 28 rpm 150 ํ C, and air. Vvm 1. before you fill the precursor (colchicine and thiocolchicine) now propeller tip speed is 3140 cm/min then increase the speed gradually end 4710 centimeters/minute after 36 hours of fermentation speed of rotor end unchanged until 72 hours of fermentation, has ended, there will be the quantity of glucose + glee and a precursor (colchicine and thiocolchicine) of 70l in 72 hours is 750, 350 g, respectively, by the peristaltic pump, coupled with automatic pH probe.
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