3. then follow the Streak end loop fuel onto a Petri dish with agar, who has already prepared with care, which includes the following steps.Petri dish, cover, 3.1, there are enough spaces that are easy to access loop, as shown in Figure 1-7 (a), and complies with Loop that bacteria are making streak at no. 1 by area during the streak will not make the food's surface crack.3.2 when the streak at no. 1, and the area, leading the Loop came out sterilization by flames after it makes a loop cooling by tapping on the edge of the area near the agar No. 1.Petri dish rotation 3.3, is in the appropriate position for the number 2 and then streak area make cross streak by beginning to address that area number 1, and then run the Streak at no. 2 area as shown at. 1-7 (b).3.4 when streak area number 2, leading the Loop came out sterilization by flames after it makes a loop cooling by tapping on the edge of the area near the agar No. 2.Petri dish, rotating 3.5 is in the appropriate position for the streak, and then no. 3 area make cross streak by beginning to address that area number 2 and then make a Streak in which the area number 3 images. 1-7 (b), if necessary, can make the streak area No. 4.3.6 mixtur back Petri and curing, curing the infection in a temperature-controlled Cabinet is 37 degrees Celsius. Long time 24-48 hours, and then observed the growth of pathogens.
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