Three slices in total from the top,

Three slices in total from the top,middle and bottom of each cell z-stack were chosen forquantification of colocalization with Manders coefficient.Thresholds were adjusted manually to eliminate backgroundfluorescence signals. If necessary, a region of interest (ROI) was drawn to image to exclude other cells or bacteria outside the cell from the analysis. For each cell weighted arithmetic mean of coefficients from three slices were calculated. Statistical significances of colocalizations were calculated during analysis using Costes algorithm, and only coincidence probabilities of P ¼ 1.00 was taken into account.

Three slices in total from the top, MIDDLE and bottom of each Cell Z-stack were Chosen for
quantification of colocalization with Manders coefficient.


Thresholds were adjusted manually to Eliminate background
fluorescence Signals.

If necessary, a Region of interest (ROI) was drawn to. image to exclude Other cells or bacteria Outside the Cell from the Analysis.

For each Cell weighted arithmetic Mean of coefficients from Three slices were calculated.

Statistical significances of Colocalizations were calculated during Analysis using Costes algorithm, and only Coincidence probabilities of P ¼ 1.00 was taken. into account.

Three slices in total from the top middle and, bottom of each cell Z-stack were chosen for.Quantification of colocalization with Manders coefficient.Thresholds were adjusted manually to eliminate background.Fluorescence signals.If necessary a region, of interest (ROI) was drawn to image to exclude other cells or bacteria outside the cell from the. Analysis.For each cell weighted arithmetic mean of coefficients from three slices were calculated.Statistical significances of colocalizations were calculated during analysis using, Costes algorithm and only coincidence. Probabilities of P ¼ 1.00 was taken into account.