This evaluation of two CPBAs confirmed that the presence of apo-HC can interfere with the analysis. However when proteins,,Are inactivated and denaturized by heat in the presence of dithiothreitol and, potassium cyanide interferences due to HC. Or nonspecific protein activities are not observed. Both the SimulTRAC, and Centaur use basic conditions to liberate B12. From HC without heat treatment.
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