ฉIn order to verify that the mice w

ฉIn order to verify that the mice were actively infected with Leptospira, bladders were cultured up to 14 days post infection in EMJH and examined for the presence of motile Leptospira by dark field At 7 d.p.i., no leptospires were detected in the bladders of Trif−/− mice and only one WT bladder was positive for bacteria. In contrast, Leptospires were detected in bladder cultures from both groups at 14 d.p.i., indicating a failure to clear the bacteria and establishment of persistent infection in mice (Supplemental Table 1). Even though the burden was elevated in the kidneys of Trif−/− mice at 1 and 3 d.p.i. compared to WT mice, measurement of creatinine in serum and observation of kidneys did not indicate any significant physiological differences in either infected group at 1 or 3 d.p.i. (Supplemental Table 1, Data not shown, Supplemental Fig. 1). Serum Nitrite levels did not differ between WT and infected groups at 7 or 14 d.p.i., further suggesting that there were no physiological differences between WT and Trif−/− mice at 7 or 14 d.p.i. (Supplemental Fig. 1). These results suggest that chronic leptospiral infection is ultimately established in both WT and Trif−/− mice, but significant differences in weight gain and spirochete burden exhibited by the Trif−/− mice compared to WT mice at 1 and 3 d.p.i. indicate that TRIF contributes to murine host defense during leptospiral infection.

ผลลัพธ์ (แอฟริกา) 1:

F Ten einde te bevestig dat die muise aktief is besmet met Leptospira, blaas is up gekweek tot 14 dae na infeksie in EMJH en ondersoek vir die teenwoordigheid van beweeglike Leptospira deur donker veld by 7 dpi, is geen leptospires bespeur in die blaas van Trif-. / - muise en net een WT blaas was positief vir bakterieë in teenstelling, is Leptospires in blaas kulture van beide groepe waargeneem by 14 dpi, dui op 'n gebrek aan die bakterieë en vestiging van aanhoudende infeksie in muise (Laerskool Table 1) duidelik Selfs... al die las verhef in die niere van Trif - / - muise op 1 en 3 dpi in vergelyking met WT muise, het meting van kreatinien in serum en waarneming van niere nie enige beduidende fisiologiese verskille in óf besmette groep op 1 of 3 dpi (dui. Laerskool Table 1, data nie getoon, Laerskool Fig 1) Serum nitriet vlakke het nie verskil tussen WT en besmette groepe op 7 of 14 dpi, verder daarop dui dat daar geen fisiologiese verskille tussen WT en Trif -.. / - muise op 7 of. . 14 dpi (. Addisionele Fig 1) Hierdie resultate dui daarop dat chroniese leptospirale infeksie uiteindelik gevestig in beide WT en Trif - / - muise, maar betekenisvolle verskille in gewigstoename en spirogeet las uitgestal deur die Trif - / - muise in vergelyking met WT muise. op 1 en 3 dpi dui daarop dat TRIF dra by tot murine gasheer verdediging tydens leptospirale infeksie.