Genomic DNA was extracted from feather bulb of old and young canaries, with approximately 1 μg of DNA ob-tained from each feather bulb (final con-centration about 20 ng/μL).
PCR was performed using a specific pair of primers designed to detect the CHD-W gene of Hemispingus frontalis which is close to the canary.
Analysis of PCR products of canary CHD-W gene on agarose gel revealed two fragments of about 345 and 306 bp for female canaries, but only a single fragment of 306 bp for male canaries (Fig. 1).
These