Changes in livestock operations over the past decade have led to concern over the increased number of Concentrated Animal Feeding Operations (CAFOs) in the United States. These livestock facilities can impair surface and groundwaters with high levels of nitrogen and pathogens. Utilizing geostatistical simulations of fecal coliform levels, this study aimed to assess the impact of livestock operations on prevalence of Escherichia Coli in Kansas between 1997 and 2003. Fecal coliform levels were negatively associated with prevalence of E. Coli. in all years, although only reaching significance in 1998. Empirical Bayes estimates indicated higher prevalence dominating the western part of the state. The negative association with E. Coli suggest that prolonged exposure to high fecal coliform levels may be protective against infection. Other studies have indicated that previous exposure to E. Coli. may result in partial resistance or complete immunity to subsequent infection.