There must be%GC content > 50% up
2.? Nucleotide sequence is a short line
(oligonucleotide), which should not exceed 18-24 NT
3. Should be the conserve region of the
ต้องการ. Study 4
. Tm should not be too high or too low
Requires% GC content > 50% up to
2 nucleotide sequence is a short cable
.(oligonucleotide) which should not exceed 18-24 NT
3. Conserve region should be the area of the
4 study. Tm. Should. Not be too high or low.
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