ประเพณี

ประเพณี

ผลลัพธ์ (อังกฤษ) 1:

Tradition.

ผลลัพธ์ (อังกฤษ) 3:

3. Amplification and sequencing PCR was performed using a thermal cycler (Model 9700; Applied Biosystems, Foster City, CA, USA), and amplified products were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems) and an ABI Prism 3100 Genetic Analyzer (Applied Biosystems). Primer sequences used in this study were the same as those given in the Clinical and Laboratory Standard Institute (CLSI) guidelines: (I) sequences of the ITS region covering ITS1, 5.8S, and ITS2 were amplified using ITS-1 / ITS-4 and ITS-5 / ITS-4 primer sets; (II) sequences of the D1 / D2 region were amplified using NL-1 / NL-4 primers; and (III) - β - tubulin gene sequences were amplified using Bt2a / T1 primers.