3.4.3 starts from the leading transport 0.2 mL solution from food Cooked in meat stock volume of 20 mL at 10 g/supplemented with glucose stimulation with L and heat (Heat shock) temperature of 75 ºC for a period of 2 minutes, followed by a boost in ice slurry. 1.5 min, and then brought to the incubator at 35 ºC for a period of 17-, 18-hour, or until you see the creation of gas occurs after taking a severe infection 2 mL 100 mL bottles that are posted on the food for the fermentation process to be used in the next step enter acetobutylicum bacteria by Cl. concentration and CFU/mL, and cassava.
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