Cell cultureHuman breast adenocarci

Cell culture

Human breast adenocarcinoma MCF7 (ATCC® HTB-22™) purchased from American Type Culture Collection (ATCC, Manassas, VA) was cultured in Roswell Park Memorial Institute (RPMI) 1640 (Lonza, Basel, Switzerland) supplemented with 10 % fetal bovine serum. Penicillin-streptomycin of 1 % was added during assays to prevent contamination. Cells were maintained in a humidified atmosphere of 5 % CO2 at 37 oC and sub-culturing was performed every three to four days depending on the confluence state.

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Cell Culture Human Breast adenocarcinoma MCF7 (ATCC® HTB-22 ™) Purchased from American Type Culture Collection (ATCC, Manassas, VA) was in Roswell Park Memorial Institute Cultured (RPMI) 1,640th (Lonza, Basel, Switzerland) supplemented with 10% fetal. bovine serum. Penicillin-streptomycin of 1% was added during assays to prevent contamination. Cells were maintained in a humidified atmosphere of 5% CO2 at 37 oC and sub-culturing was performed every three to four days depending on the confluence state.

ผลลัพธ์ (อังกฤษ) 3:

Cell culture

Human breast adenocarcinoma MCF7 (ATCC apertium HTB-22 ™) purchased from American Type Culture Collection (ATCC Manassas,,, VA) was cultured in Roswell Park Memorial Institute (RPMI) 1640 (,,) Lonza Basel Switzerland supplemented with 10% fetal. Bovine serum. Penicillin-streptomycin of 1% was added during assays to prevent contamination.Cells were maintained in a humidified atmosphere of 5% CO2 at 37 oC and sub-culturing was performed every three to four. Days depending on the confluence state.