2. ExperimentalAll experiments were

2. Experimental
All experiments were performed at 30 ± 2 ◦C apparatus.
2.1. Apparatus
A thin layer chromatographic applicator (Toshniwal, India),
20 cm × 3 cm glass plates and 24 cm × 6 cm glass jars were
used for the development of chromatographic plates. A
glass sprayer was used to spray reagent on the plate to
detect the spot. UV instrument (Model S.L.W., Advance
Research Instruments Co., India) was used for the fluorescence
studies.
2.2. Chemicals and reagents
Silica gel ‘G’, silica gel 60 F254 HPTLC plates, boric
acid, sodium hydroxide, ninhydrin, ammonium nitrate, trioctylamine
and methanol (Merck, India); sodium dodecyl sulphate
(SDS) and diphenylamine (BDH, India); amino acids, cetylpyridinium
chloride (CPC), N-cetyl N,N,N-trimethyl ammonium
bromide (CTAB), triton X-100 (TX-100) ammonium chloride,
ammonium sulphate, cobalt chloride, copper sulphate,
zinc nitrate and mercuric chloride (CDH, India); ammonium
thiocyanate, m- and p-phenylenediamine (LOBA-Chemie,
India); tri-n-butylamine and tert-butylamine (S.D. Fine Chemicals
Ltd., India) and phosphoric acid (Glaxo, India), were
used.

ผลลัพธ์ (แอฟริกา) 1:

2. eksperimentele
Alle eksperimente is uitgevoer op 30 ± 2 ° C Apparaat.
2.1. Apparaat
'n Dun lagie chromatografiese toediener (Toshniwal, Indië),
20 cm × 24 cm × 3 cm en 6 cm glas plate Glas is
wat gebruik word vir die ontwikkeling van die chromatografiese plate. A
Glass spuit is gebruik om op die bord Spray reagens om
die spot te spoor. UV instrument (Model SLW, Advance
Ondersoek Tools Co, Indië) is gebruik vir die fluoressensie
Studies.
2.2. Chemikalieë en reagense
Silica gel 'G', silika gel 60 F254 HPTLC plate, boor
suur, natriumhidroksied, Ninhidrien, ammoniumnitraat, Trioctylamine
en metanol (Merck, Indië); Dodecyl natriumsulfaat
(SDS) en difenielamien (BDH, Indië); aminosure, cetylpyridinium
chloried (koste), N-Cetyl N, N, N-Trim ammonium
bromide (CTAB), Triton X-100 (TX-100) ammoniumchloried,
ammoniumsulfaat, Cobalt chloride, Kopersulfaat,
sink nitraat en mercuric. chloried (CDH, Indië); ammonium
thiocyanate en M-P-fenyleendiamine (LOBA-Chemie,
Indië); TRI-n-butylamine en tersiêre-butylamine (SD Fine Chemicals
Ltd, Indië) en fosforsuur (Glaxo, Indië), is
gebruik.