The principle of a UV-VIS SpectrophotometerUV-VIS Spectrophotometer is an instrument used for measuring how much light intensity and UV range, and the range of white light that passes through or is being suck by examples that are placed in the tool. The wavelength of light is related to the quantity and type of the substance in the samples, most of which are organic compounds. Complex compounds and inorganic compounds that can absorb light in the wavelength range.The ability to absorb light of the substance when the sample molecules are screened with appropriate power light will cause the electrons within the atoms to absorb light and then change the status to a higher energy level in the layer when making a measurement of the amount of light passing through or reflected from the sample compared to the light from the source wavelength values according to the rules of Beer-Lambert to absorb light (absorbance) of the substance, with the number of molecules that absorb light, so this technique can be used in specifying the type and the quantity of the substance that is present in the sample.
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