Briefly mice were, deeply anesthetized using a ketamine / xylazine / acepromazine mix and intracardially perfused with 4 ° C. Slicing solution containing (in mM), 93 NMDG 93 HCl 30, glucose, NaHCO3 25, HEPES 20, MgSO4 5 10, sodium ascorbate 2.5 KCl 3 sodium,,, ,, pyruvate 2 thiourea 1.2 NaH2PO4 and 0.5 CaCl2 (pH 7.3 - 7.4 continuously oxygenated, with 95% O2 / 5% CO2).
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