AbstractBackground: With malaria dr

Abstract
Background: With malaria drug resistance increasing in prevalence and severity, new technologies are needed to
aid and improve the accuracy and clinical relevance of laboratory or field testing for malaria drug resistance. This
study presents a method based on simple and reagentless spectroscopic measurements coupled with
comprehensive spectral interpretation analysis that provides valuable quantitative information on the
morphological and compositional responses of Plasmodium falciparum and infected red blood cells (IRBCs) to
anti-malarial treatment.
Methods: The changes in the size, internal structure, nucleotide and haemozoin composition of the parasites as
well as the morphology (size and shape) and haemoglobin composition of the IRBCs treated with
dihydroartemisinin (DHA) and mefloquine (MFQ) were investigated using a spectral interpretation analysis.
Results: DHA treatment reduced the sizes of the parasites and their structural organelles. The haemoglobin
composition of the host IRBCs determined from spectroscopic analysis changed negligibly following DHA
treatment. MFQ treated parasites grew to the same size as those from parallel non-treated cultures but lacked
haemozoin. Lesser deformation of the cell shape and no haemoglobin depletion were detected for the IRBCs of
MFQ treated cultures.
Conclusions: The spectroscopic analysis method proved to be sensitive for recognition of the effects of
anti-malarial treatment on the structure and composition of the parasites and IRBCs. The method can have
significant potential for research and clinical applications such as evaluating patient specimens for drug action, drug
effects or for therapeutic monitoring.
Keywords: Malaria, Antimalarial, Spectroscopy, Spectral interpretation

ผลลัพธ์ (อังกฤษ) 3:

Abstract Background: With malaria drug resistance increasing in prevalence and severity, new technologies are needed to improve the accuracy and
aid and clinical relevance of laboratory or field testing for malaria drug resistance. This
study presents a method based on simple and reagentless spectroscopic measurements coupled with
comprehensive spectral analysis interpretation that provides valuable information on the quantitative morphological and compositional
Plasmodium responses of falciparum and infected red blood cells (IRBCs) to
anti-malarial treatment.
Methods: The changes in the size, internal structure, and haemozoin nucleotide composition of the parasites as
well as the morphology (size and shape) and composition of the haemoglobin IRBCs
treated with dihydroartemisinin (DHA) and mefloquine (MFQ) were investigated using a spectral interpretation analysis.
Results: DHA treatment reduced the sizes of the parasites and their structural organelles. The haemoglobin
composition of the host IRBCs determined from spectroscopic analysis changed negligibly DHA
following treatment. MFQ treated parasites grew to the same size as those from parallel non-treated cultures but lacked
haemozoin. Lesser deformation of the cell shape and no haemoglobin were detected for the depletion of
IRBCs MFQ treated cultures.
Conclusions:The spectroscopic analysis method proved to be sensitive for recognition of the effects of
anti-malarial treatment on the structure and composition of the parasites and IRBCs.
The method can have significant potential for research and clinical applications such as evaluating patient specimens for drug action, drug effects or for therapeutic monitoring
.
Keywords: Malaria, Antimalarial,Spectroscopy, Spectral interpretation