The objective of this study was to

The objective of this study was to develop a rapid, sensitive, and specific analytical method to study vitamin
K1 in fruits and vegetables. Accelerated solvent extraction and solid phase extraction was used for
sample preparation. Quantification was done by liquid chromatography tandem mass spectrometry with
atmospheric pressure chemical ionization in selected reaction monitoring mode with deuterium-labeled
vitamin K1 as an internal standard. The precision was estimated as the pooled estimate of three replicates
performed on three different days for spinach, peas, apples, banana, and beetroot. The repeatability was
5.2% and the internal reproducibility was 6.2%. Recovery was in the range 90–120%. No significant difference
was observed between the results obtained by the present method and by a method using the same
principle as the CEN-standard i.e. liquid–liquid extraction and post-column zinc reduction with fluorescence
detection. Limit of quantification was estimated to 0.05 lg/100 g fresh weigh

ผลลัพธ์ (แอฟริกา) 1:

Die doel van hierdie studie was om 'n vinnige, sensitief en spesifieke analitiese metode om Vitamien Bestudeer ontwikkel
K1 in vrugte en groente. Oplosmiddel ekstraksie en versnelde vaste fase onttrekking is gebruik vir
monster voorbereiding. Vloeistofchromatografie tandem kwantifisering is gedoen deur massaspektrometrie met
ionisasie in geselekteerde Atmosferiese druk Chemiese Monitoring af met deuterium gemerk
Vitamien K1 as 'n interne standaard. Die Precision geskat as die gepoelde Skat van die Drie herhalings
uitgevoer op verskillende dae vir Drie spinasie, ertjies, appels, Banana, en beet. Die herhaalbaarheid was
5,2% en die interne reproduseerbaarheid was 6,2%. Herstel was in die reeks 90-120%. Geen beduidende verskil
is waargeneem tussen die resultate verkry deur die huidige metode resultate en deur 'n metode met behulp van dieselfde
beginsel as die CEN-standaard IE Liquid-vloeistof ekstraksie en sink Vermindering Post-kolom met fluoressensie
opsporing. Perk van kwantifisering is na raming 0,05 lg / 100 g vars weeg.