Potato spindle tuber viroid (PSTVd)

Potato spindle tuber viroid (PSTVd) is an emerging pathogen that primarily affects members of the Solanaceae.
The distribution of this pathogen is expanding rapidly with several countries reporting new infections in recent years.
This pathogen is of phytosanitary concern because infected seed is thought to be one of the factors contributing
to the spread of this disease. Thus we developed a qRT-PCR method to determine the seed infection frequency
of PSTVd in tomato, as well as a RT-PCR method to determine the frequency of transmission of this pathogen to
tomato seedlings. Infection frequency of PSTVd in tomato seeds from infected plants (cv. ‘Beefsteak’) ranged from
62.3-69% and frequency of PSTVd transmission to tomato seedlings was 50.9%. Germination of the infected seed
was significantly lower (53%) than the parent seed (98%), and we also determined that viroid titers in the infected
seeds were significantly lower (average 173 ng/μl) than in the infected seedlings (785 ng/μl). Interestingly ~60% of
the infected seedlings did not display any observable symptoms of PSTVd until the second or third week; however,
plants that were grown to maturity developed severe symptoms, suggesting that a grow-out assay might not be an
accurate method for detection of this pathogen.

ผลลัพธ์ (อังกฤษ) 2:

Potato spindle Tuber viroid (PSTVd) is an emerging pathogen that primarily affects Members of the Solanaceae.
The Distribution of this pathogen is expanding rapidly with several countries Reporting New infections in recent years.
This pathogen is of Phytosanitary Concern because infected Seed is thought to be. one of the Contributing factors
to the spread of this disease. QRT-PCR thus we developed a method to Determine the frequency Seed infection
of PSTVd ​​in Tomato, RT-PCR as well as a method to Determine the frequency of Transmission of this pathogen to
Tomato seedlings. Infection frequency of PSTVd ​​in Tomato Plants Seeds from infected (CV. 'Beefsteak') ranged from
from 62.3 to 69% and frequency of PSTVd ​​Tomato seedlings was 50.9% Transmission to. Germination of the infected Seed
was significantly Lower (53%) than the parent Seed (98%), and we also determined that viroid titers in the infected
Seeds were significantly Lower (average 173 NG / Μl) than in the infected seedlings (785 NG. / μl). Interestingly ~ 60% of
the infected seedlings did not any observable symptoms of PSTVd ​​Display until the third or Second Week; however,
Grown Plants that were developed to Maturity severe symptoms, suggesting that a Grow-out assay Might not be an
Accurate method for detection of this pathogen.

ผลลัพธ์ (อังกฤษ) 3:

Potato spindle tuber viroid (PSTVd) is an emerging pathogen that primarily affects members of the Solanaceae.
The distribution. Of this pathogen is expanding rapidly with several countries reporting new infections in recent years.
This pathogen is. Of phytosanitary concern because infected seed is thought to be one of the factors contributing
to the spread of this disease.Thus we developed a qRT-PCR method to determine the seed infection frequency
of PSTVd in tomato as well, as a RT-PCR method. To determine the frequency of transmission of this pathogen to
tomato seedlings. Infection frequency of PSTVd in tomato. Seeds from infected plants (CV. 'Beefsteak') ranged from
62.3-69% and frequency of PSTVd transmission to tomato seedlings. Was 50.9%.Germination of the infected seed
was significantly lower (53%) than the parent seed (98%), and we also determined that. Viroid titers in the infected
seeds were significantly lower (average 173 ng / thermal L) than in the infected seedlings (785 ng / thermal L).? Interestingly ~ 60% of
the infected seedlings did not display any observable symptoms of PSTVd until the second or third. Week,; however
.Plants that were grown to maturity developed, severe symptoms suggesting that a grow-out assay might not be an
accurate. Method for detection of this pathogen.
.