The Other microorganisms were Grown in nutrient Broth at 35? C for 18 h.
All test microorganisms were enumerated in nutrient Broth or Malt Extract Broth by Serial dilution Method. Their final cell concentrations were 106-107 cfu / mL. The agar Diffusion Method (Sagdic, the 2,003th; Sagdic, Aksoy, & Ozkan, 2006) was Used to Detect antimicrobial Activity.
Each Microorganism (250 mL) was added Into a flask containing 25 mL STERILE nutrient agar or Malt Extract agar at 45? C. and poured into Petri dishes (9 cm diameter).
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