The framework of this work is to extract DNA from bacteria from selected soil samples of the University of Malaysia, extract all six samples from the visual enzyme indexes named in the sample collection place, namely s7a-1, s7a-11, f-14, f-1, f-110 and f-115, and use the primers specific for 16s rRNA genes of these bacteria (universal 16s rRNA primers) for PCR amplification.
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