Apple PPO was extracted from an app

Apple PPO was extracted from an apple acetone powder
according to Yoruk, Hogsette, Rolle, and Marshall (2003). Enzyme
activity was determined spectrophotometrically using chlorogenic
acid as the substrate. The 3 mL standard reaction mixture, prepared
in a cuvette, contained 2.35 mLof 0.05 mol L1 acetate buffer,pH5.2,
0.3 mL inhibition stock solution (or acetate buffer for the control),
0.3 mL of 20 mmol L1 chlorogenic acid solution prepared in the
same acetate buffer, and 0.05 mL PPO extract. The reference cuvette
contained only the chlorogenic acid solution and acetate buffer. The
changes in absorbance at 420 nm and 25
C for 2 min were
measured using a Shimadzu PharmaSpec UV-1700 UVevis spectrophotometer
(Shimadzu Scientific Instruments, Inc., Columbia,
MD, USA). The enzyme activity was determined based on the initial
reaction rates. The inhibition of PPO activity was expressed as
percent inhibition which was calculated by the formula:ฉันรักการแปล

ผลลัพธ์ (อังกฤษ) 1:

Apple PPO was extracted from an apple acetone powderaccording to Yoruk, Hogsette, Rolle, and Marshall (2003). Enzymeactivity was determined spectrophotometrically using chlorogenicacid as the substrate. The 3 mL standard reaction mixture, preparedin a cuvette, contained 2.35 mLof 0.05 mol L 1 acetate buffer,pH5.2,0.3 mL inhibition stock solution (or acetate buffer for the control),0.3 mL of 20 mmol L 1 chlorogenic acid solution prepared in thesame acetate buffer, and 0.05 mL PPO extract. The reference cuvettecontained only the chlorogenic acid solution and acetate buffer. Thechanges in absorbance at 420 nm and 25 C for 2 min weremeasured using a Shimadzu PharmaSpec UV-1700 UVevis spectrophotometer(Shimadzu Scientific Instruments, Inc., Columbia,MD, USA). The enzyme activity was determined based on the initialreaction rates. The inhibition of PPO activity was expressed asPercent inhibition was calculated by the formula: which I love the translation.

ผลลัพธ์ (อังกฤษ) 2:

Apple PPO was extracted from an Apple Acetone Powder
according to Yoruk, Hogsette, Rolle, and Marshall (the 2,003th). Enzyme
Activity was determined Spectrophotometrically using chlorogenic
acid as the substrate. The 3 mL standard Reaction mixture, prepared
in a cuvette, Contained two thirty-five MLof. twelve five mol L? 1 acetate buffer, PH5.2,
0.3 mL inhibition Stock Solution (or acetate buffer for the Control),
0.3 mL of 20 MMOL L? 1 chlorogenic acid Solution prepared in the
Same acetate buffer, and twelve five mL PPO Extract. the reference cuvette
Contained only the chlorogenic acid and acetate buffer Solution. The
changes in absorbance at 420 NM and 25? C for 2 min were
measured using a Shimadzu UV-PharmaSpec UVevis in 1700 spectrophotometer
(Shimadzu Scientific Instruments, Inc., Columbia,
MD,. USA). The enzyme Activity was determined based on the Initial
Reaction Rates. The inhibition of PPO Activity was expressed as
percent inhibition which was calculated by the Formula: I love the translation.

ผลลัพธ์ (อังกฤษ) 3:

Apple PPO was extracted from an apple acetone powder
according to Yoruk Hogsette Rolle and,,, Marshall (2003). Enzyme
activity. Was determined spectrophotometrically using chlorogenic
acid as the substrate. The 3 mL standard, reaction mixture prepared
in. A cuvette contained 2.35, mLof 0.05 mol L  1, acetate buffer pH5.2
0.3, mL inhibition stock solution (or acetate buffer for. The control),
0.3 mL of 20 mmol L  1 chlorogenic acid solution prepared in the
same acetate buffer and 0.05, mL PPO extract. The reference. Cuvette
contained only the chlorogenic acid solution and acetate buffer. The
changes in absorbance at 420 nm and 25
C for 2 min. Were
measured using a Shimadzu PharmaSpec UV-1700 UVevis spectrophotometer
(Shimadzu, Scientific Instruments Inc, Columbia
MD,,, USA).The enzyme activity was determined based on the initial
reaction rates. The inhibition of PPO activity was expressed as
percent. Inhibition which was calculated by the formula: I love the translation.