Yeast-like cells used to generate thermal destruction data were prepared by streaking M. Circinelloides onto acidified. PDA. Plates were incubated at 25 ° C for at least 48 h in a GasPack anaerobic jar (Becton Dickinson and, Co. Franklin Lakes,,, NJ). Typical colony and cellular morphology was observed following incubation. Following extraction from.GasPack anaerobic jars yeast-like cells, were immediately used for thermal destruction experiments.
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