In order to make the results more accurate that the iso-citric was made by using NADPH enzymatic method for iso-citric change in the X-ketoglutaric. acid is used to make interaction with the enzyme, iso-citric keep in mind that the volume of iso-citric acid is citric acid volumes 5-8 of total production. It also said the ammonium was detected in the culture medium. After the removal of biomass removed.
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