Hydrogel synthesis can be summarize

Hydrogel synthesis can be summarized in four main steps: (a) preparation of the starting macromer solution in the proper solvent to avoid untimely dissolution of the porogen, (2b) proper mixing with the porogen, (c) photocrosslinking reaction to form the hydrogel, and (d) dissolution of the porogen. Procedures described previously were followed to fabricate the photopolymerized hydrogels [10,16,26,27]. First, 30% (w=v) PEGDA solutions in dichloromethane (for water-soluble porogen. Procedures described previously were followed to fabricate the photopolymerized hydrogels [10,16,26,27]. First, 30% (w=v) PEGDA solutions in dichloromethane (for water-soluble porogens: salt and gelatin) or water (par-affin) were prepared. Irgacure 2959 photoinitiator was dis-solved in a 70=30v=v ethanol–water mixture, and added at a 0.5% (w=w) concentration related to the PEGDA weight. In the case of the porogen template, it was immersed in the solution and vacuum was applied to ensure good pen-etration of the macromer solution. After that, photocros-slinking was carried out with a 365-nm UV light (Fisher Scientific, Madrid, Spain) at an intensity of 4 mW=cm2 for 5min. Finally, the porogen or porogen-template was dis-solved in water (salt and gelatin) or first hexane and then water-cleaned (paraffin). To ensure complete removal of the porogen, several washings under vacuum were per-formed over a period of 1 week, and the samples were stirred in an orbital shaker.

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Irgacure 2959 photoinitiator was dis-solved in a 70 = 30V = V ethanol - water mixture and added, at a 0.5% (w = w concentration.) Related to the PEGDA weight. In the case of the, porogen template it was immersed in the solution and vacuum was applied. To ensure good pen-etration of the macromer solution. After that photocros-slinking was, carried out with a 365-nm UV light. (Fisher Scienti fi C Madrid,,Hydrogel synthesis can be summarized in four main steps: (a) preparation of the starting macromer solution in the proper. Solvent to avoid untimely dissolution of the porogen, (2b) proper mixing with the porogen, (c) photocrosslinking reaction. To form, the hydrogel and (d) dissolution of the porogen. Procedures described previously were followed to fabricate the. Photopolymerized hydrogels [10 16,,Hydrogel synthesis can be summarized in four main steps: (a) preparation of the starting macromer solution in the proper. Solvent to avoid untimely dissolution of the porogen, (2b) proper mixing with the porogen, (c) photocrosslinking reaction. To form, the hydrogel and (d) dissolution of the porogen. Procedures described previously were followed to fabricate the. Photopolymerized hydrogels [10 16,,26, the 27]. First 30% (, w = V) PEGDA solutions in dichloromethane (for water-soluble porogen. Procedures described previously. Were followed to fabricate the photopolymerized hydrogels [10 16 26,,,, 27]. First 30% (w = V) PEGDA solutions in dichloromethane. (for water-soluble porogens: salt and gelatin) or water (par-af fi n) were prepared.Spain) at an intensity of 4 mW = cm2 for 5min. Finally the porogen, or porogen-template was dis-solved in water (salt and. Gelatin) or fi RST hexane and then water-cleaned (paraf fi n). To ensure complete removal of, the porogen several washings under. Vacuum were per-formed over a period of 1 week and the, samples were stirred in an orbital shaker.Irgacure 2959 photoinitiator was dis-solved in a 70 = 30V = V ethanol - water mixture and added, at a 0.5% (w = w concentration.) Related to the PEGDA weight. In the case of the, porogen template it was immersed in the solution and vacuum was applied. To ensure good pen-etration of the macromer solution. After that photocros-slinking was, carried out with a 365-nm UV light. (Fisher Scienti fi C Madrid,,