The operating conditions for a shak

The operating conditions for a shake-flask culture of Ganoderma lucidum were studied. Of the carbon sources examined, brown sugar and
malt extract most effectively promoted mycelial growth and the production of polysaccharide, while yeast extract and skim milk were the
most effective nitrogen sources. Adding safflower seed oil to the medium significantly promoted the formation of mycelium. Additionally,
supplying CaCO3 promoted both the mycelial growth and the polysaccharide production. The optimal initial pH and incubation temperature
were 6.5 and 34 ◦C, respectively. The medium composition was then optimized using a systematic method that integrated the Taguchi array
method and the steepest ascent method. The mycelium yield and the polysaccharide production were considered as two product quality
variables. A desirability function was applied to combine these two qualities as a single objective function. The steepest ascent method was
demonstrated effectively and efficiently to approach the neighborhood of the optimum. The optimum medium composition was found to be
1.88 g l−1 CaCO3, 71.4 g l−1 brown sugar, 12.1 g l−1 malt extract, 2.28 g l−1 yeast extract, 18.4 g l−1 skim milk, 3.44 g l−1 safflower seed oil,
3.96 g l−1 olive oil, pH 6.5. When the strain was cultivated in the obtained optimal medium under the optimal operating conditions, compared
to the one before this study, the mycelium formation was markedly improved from 1.70 to 18.70 g l−1. Additionally, the polysaccharide
production also increased markedly from 0.140 to 0.420 g l−1

ผลลัพธ์ (อังกฤษ) 2:

The operating conditions for a shake-flask culture of Ganoderma lucidum were studied. Sources Of The carbon examined, Brown Sugar and
Malt Extract The Most Effectively promoted mycelial growth and production of polysaccharide, while Yeast Extract and Skim Milk were The
Most Effective Nitrogen Sources. Adding safflower seed oil to the medium significantly promoted the formation of mycelium. Additionally,
supplying CaCO3 The mycelial growth and promoted Both The polysaccharide production. The Optimal Initial Incubation Temperature and pH
were 6.5 and 34 ° C, respectively. The Medium composition was then that Integrated Optimized using a Systematic Method The array Taguchi
Method and The steepest Ascent Method. The Mycelium The polysaccharide production yield and product were considered As Quality Two
variables. A desirability function was applied to combine these two qualities as a single objective function. The steepest Ascent Method was
demonstrated Effectively and efficiently to Approach The Neighborhood of The Optimum. The Optimum Medium composition was Found to be
1.88 GL-1 CaCO3, 71.4 GL-1 Brown Sugar, 12.1 GL-1 Malt Extract, 2.28 GL-1 Yeast Extract, 18.4 GL-1 Skim Milk, 3.44 GL-1 Safflower Seed Oil,.
3.96 gl-1 olive oil, pH 6.5. When The Strain was cultivated in The Optimal Medium obtained under The Optimal operating conditions, compared
to The One Before this Study, The Mycelium Formation was markedly improved from 1.70 to 18.70 GL-1. Additionally, The polysaccharide
production also increased markedly from 0.140 to 0.420 GL-1.

ผลลัพธ์ (อังกฤษ) 3:

The operating conditions for a shake-flask culture of Ganoderma lucidum were studied. Of the carbon, sources examined brown. Sugar and
malt extract most effectively promoted mycelial growth and the production, of polysaccharide while yeast extract. And skim milk were the
most effective nitrogen sources. Adding safflower seed oil to the medium significantly promoted the. Formation of mycelium.Additionally
supplying, CaCO3 promoted both the mycelial growth and the polysaccharide production. The optimal initial. PH and incubation temperature
were 6.5 and 34 education level, C respectively. The medium composition was then optimized using a systematic. Method that integrated the Taguchi array
method and the steepest ascent method.The mycelium yield and the polysaccharide production were considered as two product quality
variables. A desirability function. Was applied to combine these two qualities as a single objective function. The steepest ascent method was
demonstrated effectively. And efficiently to approach the neighborhood of the optimum. The optimum medium composition was found to be
1.88 g l −, 1 CaCO3 71.4 g l − 1, brown sugar 12.1 g l − 1, malt extract 2.28 g l − 1, yeast extract 18.4 g l − 1, skim milk 3.44 g l − 1 safflower seed. Oil
3.96, g l − 1, olive oil pH 6.5. When the strain was cultivated in the obtained optimal medium under the optimal operating. Conditions compared
to, the one before, this study the mycelium formation was markedly improved from 1.70 to 18.70 g l − 1.? Additionally.The polysaccharide
production also increased markedly from 0.140 to 0.420 g l − 1.