เตรียม D-glucose 4.5 กรัม Glycine 1

Preparation of D-glucose Sodium Glycine 1.88 grams, 4.5 g and 0.42 g bicarbonate fill distilled water 10 ml at a temperature of 80° and bake for 2 hours, the Lao (Bernarto et al., 1997), reducing the temperature of 50° ACE. Bake it for 8 hours after that pounding. Bring to dilute with distilled water, to obtain a concentration of 1, 2, 10, 20, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1200, 1400, 1600, 1800 and 2000 ppm, then measuring the absorption (Absorbance) with an absorption measurement at 475 nm maximum wavelength (Tondee, Sirianuntapiboon and 2008) created a standard graph shows the relationship between the concentration of color, melanin, Norwich, yadin (Melanoidin) synthesis and absorption (Absorbance).

Preparation of D-glucose 4.5 g Glycine 1.88 g Sodium bicarbonate 0.42 g distilled water 10 ml to bake at a temperature of 80 ° C for 2 hours (Bernarto et al., 1997) reduce the temperature of 50 ° C and bake for 8 hours. after thoroughly Be diluted with distilled water at a concentration of 1, 2, 10, 20, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1200, 1400, 1600, 1800 and 2000 ppm then. measuring the absorbance (absorbance) by measuring light absorption at a wavelength maximum of 475 nm (Tondee and Sirianuntapiboon, 2008) standard curve shows the relationship between the concentration of colored metal yard than soil (melanoidin) synthesis and values. absorbance (absorbance)