Previously, researchers have used Lct primer in the detection of qPCR method but can not increase the DNA of L. sabrazesi a group of researcher has designed a new set of primers specific for the gene. mitochondrial cytochrome b of infection L.sabrazesi; Ls6ct2-qF and Ls6ct2-qR (Macrogen) With Manual method which Oligonucleotide primers shown in Table 1.
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