Frozen tissues are embedded in OCT

Frozen tissues are embedded in OCT when transferred to membrane slides. This OCT prevents the tissue from efficiently being captured after cutting with the laser (see picture). However, every additional step, like washing the slides in PBS and Ethanol involves the risk of fluid penetrating underneath the membrane and preventing it to detach from the slide.
Is their an alternative?

ผลลัพธ์ (อังกฤษ) 2:

Frozen tissues are embedded in OCT when transferred to membrane slides. This OCT prevents the tissue from efficiently being captured after cutting with the laser (see picture). However, every additional Step, like washing the Ethanol Slides in PBS and involves the risk of Fluid Underneath penetrating the membrane and preventing it to detach from the slide.
Is their an alternative?

ผลลัพธ์ (อังกฤษ) 3:

Frozen tissues are embedded in OCT when transferred to membrane slides. This OCT prevents the tissue from efficiently being. Captured after cutting with the laser (see picture). However every step like, additional, washing the slides in PBS and. Ethanol involves the risk of fluid penetrating underneath the membrane and preventing it to detach from the slide.Is their an alternative?