ฉันรักแปลThe efficiency of gene tar

ฉันรักแปลThe efficiency of gene targeting by integration
through homologous recombination (homologous integration,
HI) in the human pathogen Cryptococcus neoformans
remains unsatisfactory. In order to achieve a much more
efficient gene targeting system in C. neoformans, a new
double knockout strain in genes involved in the nonhomologous
end joining (NHEJ) pathway was constructed.
HI frequency was elevated by as much as approximately
fivefold in the single or double knockout strains in NHEJ
genes, and the frequency depended on the gene targeted.
None of the NHEJ gene knockouts showed significant
differences in regular growth, sensitivity to DNA-damaging
drugs or UV, and virulence compared to the wild-type
control, suggesting that the NHEJ pathway does not play a
significant role in these biological stresses in C. neoformans.
It was also suggested that the genes analyzed in this
study are components of a single NHEJ pathway, as the
mutants (including the double mutant) displayed the same
phenotypes.

ผลลัพธ์ (แอฟริกา) 1:

Ek is lief vir die vertaling van die doeltreffendheid van die integrasie Gene fokus deur
homoloë rekombinasie Deur (homoloë integrasie,
HI) patogeen Cryptococcus neoformans in die Human
bly onbevredigend. Ten einde 'n baie meer bereik
doeltreffende gene fokus in C. neoformans System, 'n New
Knockout dubbel spanning in. gene wat betrokke is in die Nonhomologous
End by (NHEJ) Pathway gebou is.
HI frekwensie verhef met soveel as ongeveer
vyfvoudig in die enkel of dubbel Knockout stamme in NHEJ
gene, en die frekwensie hang af van die Gene geteiken.
Geen van die NHEJ Gene uitklophoue. toon beduidende
verskille in gereelde groei, sensitiwiteit vir DNA beskadig
Dwelms of UV, en virulensie in vergelyking met die wilde-tipe
beheer, wat daarop dui dat die NHEJ Pathway n nie speel
n belangrike rol in hierdie Biologiese spanning in C. neoformans.
Dit is ook voorgestel. dat die gene in hierdie ontleed
Studie is komponente van 'n enkele NHEJ Pathway, as die
mutante (insluitend die dubbele mutant) vertoon die Dieselfde
fenotipes.