Extract the desired test, including basil extract extracted by water. Basil extract extracted by ethanol extract 95% flower basil extract with water. And the flower extracts basil extract with ethanol or 95% ascorbic acid (Positive control).Or methanol (Negative control) were 100 microliter with 0.1 M DPPH solution number 3 ml, mix well, leaving it in the dark at room temperature for a long time 30 minutes. Then the measured absorbance at the wavelength 517 nm. Calculate the percent removal of free radicals, P, P H (DPPH radical.Activity).
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