10.2.1 sample preparation of fresh vegetables the amount of 1000 g.10.2.2 bring samples to steaming temperature Autoclave sterilization at 121° c for a period of 15 minutes. 10.2.3 weighing, for example, is divided into 30 bags per 25 g.10.2.4 fill Escherichia coli microbes referenced 25922 DMST 4212 ATCC with the count quantity and then down to. Is as follows:The standard fuel fill Low Level 10.2.4.1 down to 3450 viable cell/ml (using the fuel Dilution 10-5 1.0 ml volume), for example, 10 bags.10.2.4.2 Medium Level to fill a standard fuel cell/ml viable 8620 (that fuel Dilution 10-5 2.5 ml volume), for example, 10 bags. 10.2.4.3 High Level standard to fill fuel cell/ml viable 24150 (10-5, that fuel Dilution Volume 7 ml) number samples 10 bags.
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