AB STR ACTObjective: To examine the

AB STR ACT
Objective: To examine the effect of Pandanus amaryllifolius (P. amaryllifolius) leaf extract on the insulin resistance
state in obese ICR mice.
Methods: Obesity was induced in mice fed with high-fat diet (45% fat) for 12 weeks. After the first six weeks on the
diet, the obese mice were administered with the water extract of P. amaryllifolius leaf at 125 and 250 mg/kg/day,
respectively for another six weeks. At the 5th week of treatment, oral glucose tolerance test was conducted. After six
weeks of treatment, the levels of blood glucose, serum insulin, leptin, adiponectin, and lipid profiles were determined.
The liver, muscle and epididymal fat tissues were removed for measuring the biochemical parameters and protein
expression, as well as histological examination.
Results: Six weeks of treatment with P. amaryllifolius led to a significant reduction in the blood glucose level as well
as improvement in the insulin resistance. P. amaryllifolius also increased the liver glycogen storage and serum
adiponectin and decreased the serum leptin levels. A reduction in the serum and hepatic triglyceride, and
non-esterified fatty acid levels was also observed. The histological examination showed that the obese mice treated
with P. amaryllifolius reduced the lipid droplet in liver tissue and adipocyte size in epididymal fat tissue. The
treatment also increased the protein expression of glucose transporter 4 in the muscle and fat tissues.
Conclusions: The treatment with P. amaryllifolius could decrease several parameters of impaired glucose and lipid
metabolism. To the best of our knowledge, this is the first report on the role of P. amaryllifolius leaf extract in
alleviating the insulin dysfunction in obesity state.

ผลลัพธ์ (อังกฤษ) 2:

AB STR ACT
Objective: To examine the Amaryllifolius Effect of Pandanus (P. Amaryllifolius) Leaf Extract on the insulin resistance
in obese State ICR mice.
Methods: Obesity was induced in mice fed with High-FAT diet (45% FAT) for 12 weeks. . After the First Six weeks on the
diet, the obese mice were administered with the Water Extract of P. Leaf Amaryllifolius at 125 and 250 mg / kg / Day,
for another Six weeks respectively. At the 5th week of treatment, oral glucose tolerance test was conducted. Six after
weeks of Treatment, the Blood levels of glucose, serum insulin, leptin, adiponectin, and lipid profiles were determined.
The liver, and epididymal FAT Muscle tissues were removed for measuring the Biochemical Parameters and protein
Expression, as well as histological Examination.
results: Six weeks of Treatment with P. Amaryllifolius LED to a significant Reduction in the Blood glucose level as well
as the Improvement in insulin resistance. Increased liver glycogen also the Amaryllifolius P. Storage and serum
adiponectin and leptin levels Decreased the serum. A Reduction in the serum and hepatic triglyceride, and
non-esterified fatty acid levels was also observed. Showed that the obese mice the histological Examination treated
with P. Amaryllifolius reduced in the liver tissue and adipocyte lipid Droplet Size in epididymal tissue FAT. The
Treatment Expression of glucose transporter protein also Increased the 4 in the FAT and Muscle tissues.
Conclusions: Treatment with P. The Amaryllifolius could decrease glucose and lipid several Parameters of impaired
metabolism. To the best of our Knowledge, this is the First Report on the role of P. Leaf Extract Amaryllifolius in
alleviating the insulin dysfunction in obesity State.