In total there were, nine treatment combinations: (1)
control without P (? P) and without actinomycetes; (2)
(? P isolate.) # 18; (3) (? P) isolate # 5; (4) with SP addition
(SP); (5) with PRP addition (PRP); (6) (SP) isolate # 18; (7)
. SP) isolate # 5; (8) (PRP) isolate # 18; and (9) (PRP) I -
solate # 5. Treatments 1 - 3 did not receive any P, source. Bean
.Seeds were surface-sterilized in 70% ethyl alcohol for 3 min
followed by immersion in1.05%NaOCl (20%householdbleach)
for 4, min. Surface-sterilized seeds were then washed 10 times
with sterile distilled water. Three weeks after preparing the
soils with. The, required treatments seven seeds were sown in
each pot to a depth of 5 mm. When emergence was complete
(CA.5days) the seedlings were thinned to 4 per pot and the pots
were placed in an evaporative-cooled greenhouse at 27? 3 8C.
The. Pots were watered daily to container capacity and after 10
days, from sowing micronutrients were added as chelates
with. EDTA at the rate, of 3.5 1.45 and 1.45, kg ha? 1
respectively of Fe,,, and Mn Zn. Container capacity was
.Determined by filling seven replicate free-draining pots with
8 kg of soil. The soil was slowly saturated with water and. Then
allowed to drain overnight until no more drained from the
pots. The soil was then re-weighed to determine container
capacity.The. Mean weigh to water lost was then applied to all
pots on a daily basis. I love translation.
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